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“TwistAmp Basic RT试剂盒”参数说明
| 是否有现货： | 是 | 认证： | TwistDX |
| 类型： | 核酸 | 型号： | TABSRT01KIT |
| 规格： | 96次 | 商标： | Twistdx |
| 包装： | 纸盒 |
“TwistAmp Basic RT试剂盒”详细介绍
TABSRT01KIT TwistAmp™ Basic RT 用于逆转录RPA反应
产品描述：
TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp Basic用于逆转录反应，CDNA快速扩增. 除基本组分外, 还加有通用的逆转录酶，但不含Rnase抑制剂。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
产品描述：
TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp Basic用于逆转录反应，CDNA快速扩增. 除基本组分外, 还加有通用的逆转录酶，但不含Rnase抑制剂。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
